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KMID : 0545120020120010153
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 1 p.153 ~ p.156
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Cellulase-Free Thermostable Alkaline Xylanase from Thermophilic and Alkalophilic Bacillus sp. JB-99
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JOHNVESLY, B.
VIRUPAKSHI, S./PATIL, G. N./RAMALINGAM/NAIK, G. R.
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Abstract
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The characterization of a partially purified, cellulase-free, thermostable alkaline xylanase from thermoalkalophilic Bacillus sp. JB-99 was investigated. The xylanase production was the highest when birchwood xylan was added to a medium containing finely powdered rice bran, showing 4,826 IU ml^-1 of activity for 15 h of incubation. The partially purified xylanase exhibited an optimum temperature and pH at 70℃ and 10, respectively. The enzyme was stable at pH 5-11 at 50℃. The xylanase activity was strongly inhibited by Hg^2+, while dithiothreitol, cysteine, and β-mercaptoethanol enhanced the activity.
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